Glucose-6-phosphatase in normal adult human intestinal mucosa.
نویسندگان
چکیده
1. The existence of specific glucose-6-phosphatase activity in human intestinal mucosa has been somewhat controversial. 2. We have demonstrated the presence of low levels of specific glucose-6-phosphatase activity in normal human adult intestinal mucosa. Activity was found in oesophagus, stomach, duodenum and colon. 3. Immunoblot analysis using antibodies monospecific for the 36.5 kDa liver glucose-6-phosphatase catalytic subunit demonstrated that intestinal mucosa contains low levels of the glucose-6-phosphatase enzyme protein. 4. The low levels of activity together with problems of proteolysis make human intestinal biopsies unsuitable for use in the diagnosis of type 1 glycogen-storage disease.
منابع مشابه
An investigation into the enzyme histochemistry of adenocarcinomas of human large intestine and of the transitional epithelium immediately adjacent to them.
Histochemical enzymatic studies were performed on 30 freshly resected large bowel carcinomas, 30 samples of normal colonic epithelium, and six samples of the histologically normal epithelium (so-called transitional epithelium) immediately adjacent to a carcinoma. Five enzymes were studied: nicotine adenine dinucleotide tetrazolium reductase (NADH-TR), glucose-6-phosphate dehydrogenase, succinat...
متن کاملThe metabolism of glucose 6-phosphate in developing mammalian tissues.
Two marked changes in the metabolism of glucose 6-phosphate are known to occur in the liver during mammalian development before maturation. First, hepatic glucose 6-phosphatase (D-glucose 6phosphate phosphohydrolase, EC 3.1.3.9) appears late in gestation and increases rapidly after birth in several species (Nemeth, 1954; Weber & Cantero, 1955; Kretchmer, 1959; Coquoin-Carnot & Roux, 1960a; Dawk...
متن کاملThe hydrolysis of glucose monophosphates by a phosphatase preparation from pea seeds.
Although many phosphatase preparations have been obtained from plant tissues there are few data available on the breakdown of glucose monophosphates, and of glucose 1-phosphate in particular. In the work of Porter (1953), Spencer (1954) and Roberts (1956), it was found that glucose 1phosphate was hydrolysed by extracts from potato tubers, tomato leaves and wheat leaves respectively; however, mo...
متن کاملMaximal activities of glutaminase and some enzymes of glycolysis and ketone body utilization and rates of utilization of glutamine, glucose and ketone bodies by intestinal mucosa after burn injury.
1. The effects of burn injury (33 per cent of body surface area) on the activities of key enzymes in the metabolism of glucose, glutamine and ketone bodies in the epithelial cells of the small intestine and the rates of utilization of glucose, glutamine and ketone bodies by isolated enterocytes have been investigated. 2. Burn injury decreased the maximal activities of hexokinase and 6-phosphofr...
متن کاملAlkaline phosphatase levels in normal and diseased small bowel.
Since the introduction of peroral biopsy of the small intestine (Shiner, 1956; Crosby and Kugler, 1957) there has been growing interest in intestinal enzymes as well as morphology. The disaccharidases have received most attention, but many other enzymes, including alkaline phosphatase, are present in the small intestinal mucosa. There are few reports on human intestinal alkaline phosphatase and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical science
دوره 83 6 شماره
صفحات -
تاریخ انتشار 1992